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Growth Curves

We are calculating the rate of growth of each cell line by counting the total number of cells in duplicate wells every day for 4 to 6 days.


BG01(1) Growth

Growth curve for BG01(1)

Days (in culture) Cells/Well Average #

of Cells
Standard

Deviation
Day 1 115000

115000

105000

105000
110000 5774
Day 2 117500

155000

152500

132500
139375 17722
Day 3 190000

237500

145000

175000
186875 38588
Day 4 380000

227500

287500

320000
303750 63656
Day 5 642500

532500

547500

690000
603125 75674
Day 6 675000

710000

885000

770000
760000 92105

UC06 Growth

Growth curve for UC06

Days (in culture) Cells/Well Average #

of Cells
Standard

Deviation
Percentage

(compared to Day 1)
Experiment 1
Day 1 157500 157500 100.00
Day 2 no data no data no data no data
Day 3 350000 350000 222.22
Day 4 640000 640000 406.35
Day 5 1290000 1290000 819.05
Experiment 2
Day 1 226000

196000
211000 21213.2 100.00
Day 2 334000

296000
315000 26870.1 149.29
Day 3 557500

517500
537500 28284.3 254.74
Day 4 997500

910000
953750 61871.8 452.01
Experiment 3
Day 1 147500

162500
155000 10606.6 100.00
Day 2 192000

208000
200000 11313.7 129.03
Day 3 337500

350000
343750 8838.8 221.77
Day 4 765000

627500
696250 97227.2 449.19
Day Average Percentage Standard Deviation
Day 1 100.00 0.00
Day 2 139.16 14.33
Day 3 232.91 18.91
Day 4 435.85 25.59

WA01 Growth

Growth curve for WA01

Days (in culture) Cells/Well Average #

of Cells
Standard

Deviation
Day 1 1475000

1500000

1025000

1300000
1325000 218898.7589
Day 2 1425000

1950000

1575000

1700000
1662500 222204.8604
Day 3 2700000

2575000

3325000

3150000
2937500 357363.0274
Day 4 3350000

3175000

2575000

3050000
3037500 331976.4048
Day 5 7800000

9400000

4950000

5400000
6887500 2090603.98
Day 6 11450000

12250000

8650000

8350000
10175000 1965324.401

WA07 Growth

Growth curve for WA07

Days (in culture) Cells/Well Average #

of Cells
Percentage

(compared to Day 1)
Experiment 1
Day 1 77500

105000

95000

97500
93750 100
Day 2 70000

70000

87500

75000
75625 80.67
Day 3 165000

180000

185000

152500
170625 182
Day 4 185000

182500

210000

240000
204375 218
Day 5 417500

487500

372500

455000
433125 462
Day 6 No data No data No data
Experiment 2
Day 1 925000

750000

600000

925000
800000 100
Day 2 1250000

1050000

475000

1125000
975000 121.88
Day 3 1600000

1675000

1800000

2175000
1812500 226.56
Day 4 4800000

4650000

4450000

3325000
4306250 538.28
Day 5 6350000

6450000

6650000

6350000
6450000 806.25
Day 6 7300000

8150000

7300000

7350000
7525000 940.63
Day Average Percentage Standard Deviation
Day 1 100 0
Day 2 101.27 29.14
Day 3 204.28 31.51
Day 4 378.14 226.47
Day 5 806.25 243.42
Day 6 940.63 0

WA09 Growth

Growth curve for WA09

Days (in culture) Cells/Well Average #

of Cells
Standard

Deviation
Percentage

(compared to Day 1)
Experiment 1
Day 1 146000

156000
151000 7071.1 100.00
Day 2 188000

212000
200000 16970.6 132.45
Day 3 495000

585000
540000 63639.6 357.62
Day 4 745000

532500
638750 150260.2 423.01
Experiment 2
Day 1 128000

128000
128000 0 100.00
Day 2 155000

182500
168750 40750 131.84
Day 3 174000

206000
190000 21250 148.44
Day 4 520000

537500
528750 338750 413.09
Day Average Percentage Standard Deviation
Day 1 100.00 0.00
Day 2 132.14 0.43
Day 3 253.03 147.91
Day 4 418.05 7.02