The NIH Stem Cell Unit (l-r): Kye-Yoon Park, Barbara Mallon, Rebecca Hamilton, and Kevin Chen.
To allow scientists to select which lines are most suitable for their intended experiments, the research community has consistently requested that available cell lines be more fully characterized.
To address this important need, the NIH Intramural Research Program has created the NIH Stem Cell Unit (SCU). The SCU has established a database to which hESC lines, adult stem cells and induced pluripotent stem (iPS) cells may be directly compared. These data give scientists the information they need when choosing individual cell lines for specific projects.
The Unit's main goal is to identify—and share with the research community—the similarities and differences between the hESC lines when subjected to a standardized paradigm.
- Qin H, Cho M, Ha W, Zhang L, Tasian SK, Oo HZ, Negri GL, Lin Y, Zou J, Mallon BS, Maude S, Teachey DT, Barrett DM, Orentas RJ, Daugaard M, Sorensen PH, Grupp SA, Fry TJ. Eradication of B-ALL using chimeric antigen receptor-expressing T cells targeting the TSLPR oncoprotein. Blood. 2015 Jul 30;126(5):629-39. doi: 10.1182/blood-2014-11-612903. Epub 2015 Jun 3. PMID: 26041741
- Lee CT, Bendriem RM, Kindberg AA, Worden LT, Williams MP, Drgon T, Mallon BS, Harvey BK, Richie CT, Hamilton RS, Chen J, Errico SL, Tsai SY, Uhl GR, Freed WJ. Functional consequences of 17q21.31/WNT3-WNT9B amplification in hPSCs with respect to neural differentiation. Cell Rep. 2015 Feb 3;10(4):616-32. doi: 10.1016/j.celrep.2014.12.050. Epub 2015 Jan 29. PMID: 25640183 Free Article
- Chen KG, Hamilton RS, Robey PG, Mallon BS. Alternative cultures for human pluripotent stem cell production, maintenance, and genetic analysis. J Vis. Exp. 2014 Jul 24;(89). doi: 10.3791/51519. PMID: 25077932 Free PMC Article
- Phillips MD, Kuznetsov SA, Cherman N, Park K, Chen KG, McClendon BN, Hamilton RS, McKay RD, Chenoweth JG, Mallon BS, Robey PG. Directed differentiation of human induced pluripotent stem cells toward bone and cartilage: in vitro versus in vivo assays. Stem Cells. Transl Med. 2014 Jul;3(7):867-78. doi: 10.5966/sctm.2013-0154. Epub 2014 May 22. PMID: 24855277
- Kuznetsov SA, Cherman N, Robey PG. In vivo bone formation by progeny of human embryonic stem cells. Stem Cells and Development. 2010 Sep 14 [Epub ahead of print].
- Cimato T, Beers J, Ding S, Ma M, McCoy JP, Boehm M, Nabel EG. (2009) Neuropilin-1 identifies endothelial precursors in human and murine embryonic stem cells before CD34 expression. Circulation. 119(16):2170–8.
- Xing Yan, Haiyan Qin, Cunye Qu, Rocky S Tuan, Songtao Shi, George TJ Huang. (2009) iPS cells reprogrammed from mesenchymal-like stem/progenitor cells of dental tissue origin. Stem Cells and Development. 19(4):469–80.
NIH Stem Cell Unit Citations
Kumari D and Usdin K. (2010) The distribution of repressive histone modifications on silenced FMR1 alleles provides clues to the mechanism of gene silencing in fragile X syndrome. Human Molecular Genetics. 19(23):4634–42.
- Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 131(5):861–72.