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Stem Cell Information

Karyotyping Analysis

SKY analysis courtesy of NCI

G-banding performed by Cancer Genetics Inc.

G-banding performed by Cell Line Genetics

  • BG02 at p33 was normal
  • BG03 at p81 was normal
  • ES02 at p49 was normal
  • ES04 at p76 was normal
  • ES05 at p45 was normal
  • SA01 at p24 was normal
  • TE06 at p74 was normal
  • UC01 at p46 was normal; fluorescent in situ hybridization (FISH) was performed to detect small populations of trisomy 12 and/or 17, and they were found to be normal for this also
  • UC06 at p78 was normal
  • WA07 at p66 was normal
  • WA14 at p33 was normal
  • WA13 at p10+24 was normal

BG01 SKY Images

Left image: hES chromosomes after the simultaneous hybridization of 24 combinatorially labeled chromosome painting probes. Middle image: hES chromosome were stained with dapi, which used to compare within chromosomes and banding. Right image: Presentation of spectrabased classification colors. Bottom image: Karyotype of the SKY analysis from a normal female hES cell.

Click to load the high-resolution version

BG01 Karyotype
Metaphase # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y Total Chromosome Count     Remark    
1 2 2 2 2 2 2 2 2 2 2 2 *3 2 1 2 2 3 2 2 1 2 2 2 0 16 XX, +12q!, +17, -14, -20
2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 3 2 2 2 1 2 2 1 16 XXY, +17, -21, -22
9 2 2 2 2 2 2 3 2 1 2 2 1 2 2 2 2 3 1 0 2 1 2 1 1 12 XY, +7, +17, -9, -12, -18, -19, -19, -21
10 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 1 1 2 2 0 15 XX, +17, -20, -21
11 2 2 2 2 2 1 1 2 1 2 1 2 1 1 2 2 1 2 1 2 2 1 2 1 18 XXY, -6, -7, -9, -11, 13, -14, -17, -19, -22
13 2 2 2 2 2 2 2 2 1 2 1 2 2 2 2 2 1 2 1 2 2 2 2 0 12 XX, -9, -11, -17, -19
14 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 1 2 2 1 17 XXY, +17, -21
15 2 2 2 2 3 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 1 1 1 17 XY, +5, +17, -22
16 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 2 1 18 XXY, +17
17 2 2 2 2 2 2 2 2 2 2 1 2 1 1 1 2 3 2 1 2 2 1 2 0 11 XX, t(17;22), +17, -11, -13, -14, -15, -19, -22
18 2 2 2 2 2 2 3 2 2 2 2 2 2 2 2 2 3 2 1 2 2 2 1 1 17 XY, +7, +17, -9
19 2 2 1 2 2 2 2 2 2 2 1 2 2 2 2 2 3 2 2 2 2 1 2 14 XX, +17, -3, -11, -22
20 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 4 2 2 2 2 2 2 1 19 XXY, +17, +17
21 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 2 1 18 XXY, +17
22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 1 2 1 17 XXY, +17, -21
23 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 3 2 2 2 2 2 2 1 19 XXY, +12, +17

    = Missing chromosomes
   ,    = Extra chromosomes

16 metaphases analyzable; 5 metaphases with XX, 3 metaphases with XY, 8 metaphases with XXY

* broken chromosome, or del(12p)


UC06 SKY Images

Left image: hES chromosomes after the simultaneous hybridization of 24 combinatiorially labeled chromosome painting probes. Middle image: hES chromosome were stained with dapi, which used to compare within chromosomes and banding. Right image: Presentation of spectrabased classification colors. Bottom image: Karyotype of the SKY analysis from a normal female hES cell.

Click to load the high-resolution version

UC06 Karyotype
Metaphase # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y Total Chromosome Count     Remark    
1 2 2 2 2 2 2 2 1 1 2 2 2 2 2 2 2 2 2 1 2 2 1 2 42 XX, -8,-9,-19,-22
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 45 XX, -21
3 2 2 2 2 1 2 1 2 2 2 1 2 2 1 2 1 1 2 2 1 2 2 2 37 XX
5 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 45 XX, -10
8 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
9 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
10 2 2 2 2 2 2 2 3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 47 XX, +8
11 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 2 47 XX, +17
12 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
13 2 2 2 2 *2 2 2 2 2 2 2 2 2 2 2 2 3 2 1 3 2 2 2 47 XX, +17, -19, +20
15 2 *1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 2 46 XX, -2, +17
16 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 2 46 XX, repeat cell, same as metaphase #15
17 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
19 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 2 2 2 2 1 46 XX, +17, -22
22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 45 XX, -19
23 2 2 2 2 2 2 2 3 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 46 XX, +8, -21
24 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
25 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX, -2
26 2 1 2 2 2 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 42 XX, -2, -6, 7, -8

    = Missing chromosomes
    = Extra chromosomes

20 metaphases analyzable

* broken chromosome, or del(5q)


WA01 Karyotype
Metaphase # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y Total Chromosome Count     Remark    
1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 0 2 1 0 41 X, -Y, -19, -20, -21, -21
2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
4 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
5 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 45 X, -Y
6 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
7 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
8 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 1 1 45 XY, -15
9 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
10 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
11 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
12 2 2 2 2 2 1 2 2 2 2 2 2 1 0 2 2 2 2 2 1 2 2 1 1 41 XY, -6, -13, -14, -14, 20
13 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 1 1 45 XY, -19
14 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
15 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
16 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY
17 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 1 46 XY

    = Missing chromosomes (Note: The random nature of these events suggests a technical anomaly rather than a genetic abnormality.)


WA07 SKY Images

Left image: hES chromosomes after the simultaneous hybridization of 24 combinatiorially labeled chromosome painting probes. Middle image: hES chromosome were stained with dapi, which used to compare within chromosomes and banding. Right image: Presentation of spectrabased classification colors. Bottom image: Karyotype of the SKY analysis from a normal female hES cell.

Click to load the high-resolution version

WA07 Karyotype
Metaphase # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y Total Chromosome Count     Remark    
1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
4 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 45 XX, -17
5 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
6 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
7 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
8 2 2 2 2 2 2 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 44 XX, -7, -8
9 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
10 2 2 2 2 2 1 2 2 2 2 1 2 2 2 2 2 2 2 2 1 2 2 2 43 XX, -6, -11, -20
11 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
12 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
13 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
14 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 2 2 2 2 2 2 2 45 XX, -13
15 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX

    = Missing chromosomes (Note: The random nature of these events suggests a technical anomaly rather than a genetic abnormality.)


WA09 SKY Images

Left image: hES chromosomes after the simultaneous hybridization of 24 combinatiorially labeled chromosome painting probes. Middle image: hES chromosome were stained with dapi, which used to compare within chromosomes and banding. Right image: Presentation of spectrabased classification colors. Bottom image: Karyotype of the SKY analysis from a normal female hES cell.

Click to load the high-resolution version

WA09 Karyotype
Metaphase # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y Total Chromosome Count     Remark    
1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX, SKY analyzed, metha 1-12
2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
4 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
5 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
6 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
7 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
8 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
9 2 2 2 2 2 2 2 2 1 2 1 2 2 2 2 2 2 2 2 2 1 2 2 43 XX, -9, -11, -21
10 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
11 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
12 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
13 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX, cells counted, 13-22
14 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
15 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 45 XX, -22
16 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
17 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
18 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
19 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 45 XX, -2
20 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
21 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX
22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 46 XX

    = Missing chromosomes (Note: The random nature of these events suggests a technical anomaly rather than a genetic abnormality.)