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Stem Cell Information

Immunocytochemistry of Undifferentiated Cells

These cell lines have been immunostained for appropriate ES cell markers (see "Antibody Information" chart below) and found to be positive for Oct-4, SSEA-4, Tra-1-60, Tra-1-81 and mostly negative for SSEA-1.

Antibody Information
Oct-4 1:1000 Santa Cruz sc-5279
SSEA-1 1:20 DSHB MC-480
SSEA-4 1:50 DSHB MC-813-70
Tra-1-60 1:100 Kind gift of Peter Andrews, Sheffield University, UK
Tra-1-81 1:100 Kind gift of Peter Andrews, Sheffield University, UK