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Stem Cell Information

The NIH Stem Cell Characterization Facility

Chen KG, Mallon BS, Mckay RD, Robey PG. Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics. Cell Stem Cell. 2014;14(1):13–26.

Chen KG, Mallon BS, Johnson KR, Hamilton RS, Mckay RD, Robey PG. Developmental insights from early mammalian embryos and core signaling pathways that influence human pluripotent cell growth and differentiation. Stem Cell Res. 2014;12(3):610–621.

Mallon BS, Hamilton RS, Kozhich OA, et al. Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin. Stem Cell Res. 2014;12(2):376–86.

The SCU has established a database to which hESC lines, adult stem cells and induced pluripotent stem (iPS) cells may be directly compared. These data give scientists the information they need when choosing individual cell lines for specific projects. Lines eligible for use by NIH-funded researchers under current NIH policy (per the July 7, 2009, NIH stem cell guidelines) are listed at http://grants.nih.gov/stem_cells/registry/
current.htm
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The initial aim of the SCU is to establish standards for all aspects of the culture process, as well as quality control and monitoring of these hESC lines. By performing these assays in a single laboratory, the SCU has established a database to which additional hESC lines, adult stem cells and induced pluripotent stem (iPS) cells may be directly compared. These data give scientists the information they need when choosing individual cell lines for specific projects.

Researchers in the field may contact us at nihstemcellunit@mail.nih.gov

Culture Protocols with new banner

Learn about the cell lines being analyzed, culture procedures, and assay protocols used by the SCU.

Analysis Results

Review the results of the SCU's analyses for genotyping, karyotyping, subcloning, and more.

Search Databases

Get advanced analysis and characterization results for 21 human embryonic stem cell lines.